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Cell-free translation of PV genomic RNA is unaffected by the presence of rPTP. (A) Cell-free reactions were programmed with PV replicon RNA containing luciferase in place of the capsid-coding sequence. Translation was measured after 12 h via a luciferase activity assay. (B) Cell-free reactions were programmed with PV vRNA. After 12 h of translation, products were separated via SDS-PAGE. (C) Extracts were programmed with PV vRNA, and viable virus produced was quantitated by plaque assay.
