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. 2007 Aug 8;81(20):11256–11266. doi: 10.1128/JVI.01028-07

TABLE 1.

Kinetic and thermodynamic constants for PV 3Dpol-catalyzed nucleotide incorporation

Primer and templatea Nucleotide Kd(app) (μM) kpol (s−1) kpol/Kd(app) (s−1 μM−1)
s/s-A
    GCUAGGGCCC UTPb 98 ± 2 266 ± 2 2.7
                    CCCGGGAUCG rPTP 134 ± 48 0.47 ± 0.05 0.0035
s/s-C
    GAUCGGGCCC GTPb 3.8 ± 0.7 57 ± 3 15
                    CCCGGGCUAG RTPc 430 ± 79 0.019 ± 0.002 0.000044
s/s-G
    CAUGCCCGGG CTPb 19 ± 3 157 ± 8 8.2
                    GGGCCCGUAC rPTP 132 ± 29 0.59 ± 0.05 0.0045
s/s-U
    CGAUGGGCCC ATPb 134 ± 18 86.7 ± 3.7 0.86
                    CCCGGGUACG RTPc 496 ± 21 0.014 ± 0.001 0.000028
a

The templating bases are underlined.

b

Data are based on reference 3.

c

Data are based on reference 7.