TABLE 1.
Primer and templatea | Nucleotide | Kd(app) (μM) | kpol (s−1) | kpol/Kd(app) (s−1 μM−1) |
---|---|---|---|---|
s/s-A | ||||
GCUAGGGCCC | UTPb | 98 ± 2 | 266 ± 2 | 2.7 |
CCCGGGAUCG | rPTP | 134 ± 48 | 0.47 ± 0.05 | 0.0035 |
s/s-C | ||||
GAUCGGGCCC | GTPb | 3.8 ± 0.7 | 57 ± 3 | 15 |
CCCGGGCUAG | RTPc | 430 ± 79 | 0.019 ± 0.002 | 0.000044 |
s/s-G | ||||
CAUGCCCGGG | CTPb | 19 ± 3 | 157 ± 8 | 8.2 |
GGGCCCGUAC | rPTP | 132 ± 29 | 0.59 ± 0.05 | 0.0045 |
s/s-U | ||||
CGAUGGGCCC | ATPb | 134 ± 18 | 86.7 ± 3.7 | 0.86 |
CCCGGGUACG | RTPc | 496 ± 21 | 0.014 ± 0.001 | 0.000028 |