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. 2007 Aug 8;81(20):11282–11289. doi: 10.1128/JVI.00910-07

TABLE 2.

Substitutions introduced into the HEF protein of C/JHB/1/66 and enzyme restriction strategy used to analyze the stability of the rescued mutants

Virus description Amino acid position Amino acid Nucleotide sequenceb Restriction site After digestionc
wta 284 Thr GAC ACC None 875
465 Thr ACA GGA None 875
Mutants 284 Ile GAT ATC EcoRV 694 + 181
465 Ala GCC GGC NgoMIV 727 + 148
a

Wild type.

b

Underlined, nucleotide codons coding for the indicated amino acid; in bold, nonsilent mutations; in italics, silent mutations introduced in the neighboring codons to design a restriction enzyme site.

c

Expected length(s) (nt) of fragment(s) produced after digestion of amplicons with the indicated restriction enzymes.