FIG. 4.

Viral protein synthesis in Vero cells infected with the ΔU_L49 mutant virus. Confluent cell monolayers were either mock infected (lane 1) or infected with 10 PFU/cell of either HSV-1(F) (lanes 2 to 5) or the ΔU_L49 mutant virus (isolate V1) (lanes 6 to 9). The cells were harvested at the indicated times and processed as described in Materials and Methods. Equal amounts of proteins were electrophoretically separated on a 10% denaturing polyacrylamide gel, transferred to a nitrocellulose sheet, and reacted with antibodies against representative α (ICP0), β (U_S3), and γ (γ₁, VP16 and VP22; γ₂, U_S11) proteins. An anti-actin antibody was used as a control.