TABLE 1.
Virus yieldsa in different cell lines infected with wild-type HSV-1 or ΔUL49-V1
| Cell line | Virus | Yield at the following time (h) after infection:
|
||||
|---|---|---|---|---|---|---|
| 3 | 12 | 18 | 24 | 48 | ||
| Vero | HSV-1(F) | 2.0 × 103 | 1.2 × 107 | 2.0 × 108 | 2.2 × 107 | 2.0 × 107 |
| ΔUL49-V1 | 6.0 × 102 | 5.7 × 105 | 5.6 × 107 | 1.4 × 107 | 1.2 × 107 | |
| HEp-2 | HSV-1 | 8.4 × 104 | 7.3 × 106 | 4.7 × 107 | 1.0 × 108 | 2.0 × 108 |
| ΔUL49-V1 | 1.2 × 104 | 3.6 × 105 | 1.4 × 106 | 3.1 × 106 | 1.0 × 107 | |
| HeLa | HSV-1 | 2.0 × 103 | 8.5 × 105 | 7.5 × 106 | 1.3 × 107 | 1.2 × 107 |
| ΔUL49-V1 | 2.0 × 103 | 8.7 × 104 | 5.6 × 105 | 1.3 × 106 | 1.7 × 106 | |
a
PFUs were determined by a plaque assay on Vero cell monolayers as described in Materials and Methods.