FIG. 1.

Effect of Vps4E228Q and Vps24-Red on the yield of extracellular and cell-associated HSV-1. 293T cells (A, B) and COS-7 cells (C, D) were transfected either with an empty vector (pBJ5 or pDsRed2-N1) or with constructs expressing either Vps4E228Q or Vps24-Red. Twelve hours after transfection, the cells were infected with HSV-1 at 1 PFU/cell (A, C) or 10 PFU/cell (B, D). At 36 h postinfection, the extracellular (Extracell) and cell-associated virus were titrated by plaque assay in Vero cells.