FIG. 9.

Luciferase expression in Tet-OFF MEF cultures that were mock treated (gray bars) or treated with 10 IU/ml of IFN-α/β for 3 h (hatched bars) prior to infection. Cells were harvested at 8 hpi with 39MCS-fLuc. (A) Comparison of specific siRNA pools targeting ZAP, ISG20, ISG15, and ISG56 with controls, including a cyclophilin B-specific siRNA pool, a nontargeting negative control pool, transfection reagent alone, or no treatment. (B) Comparison of specific siRNA pools targeting viperin with a nontargeting negative control pool, transfection reagent alone, or no treatment. (C) Comparison of individual siRNA pools targeting ZAP, ISG20, ISG15, or ISG56 with combinations of two siRNA pools and control treatments described for panel A above.