FIG. 10.

Effects of cleavage site mutations on RNA synthesis. Arabidopsis protoplasts were transfected with wild-type or mutant in vitro transcripts, alone or in combination with expression vectors as indicated. The cells were harvested 48 h posttransfection, and equivalent RNA amounts were analyzed based on rRNA as a loading control. Plus-strand genomic (g) and subgenomic (sg) TYMV RNAs (top) and minus-strand TYMV RNAs (bottom) were detected by Northern blotting. The relative accumulation of viral RNAs is indicated below each panel.