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. 2007 Aug 8;81(20):11402–11412. doi: 10.1128/JVI.01428-07

FIG. 4.

FIG. 4.

Mutagenesis of the proposed cleavage site generating the 115K protein. (A) Amino acid sequence alignment of the PRO/HEL and HEL/POL cleavage sites. Identical or similar residues are boxed in black or gray, respectively. The arrow indicates the previously characterized HEL/POL and the proposed PRO/HEL cleavage sites. (B) Amino acid sequence of the PRO/HEL (left) and HEL/POL (right) cleavage sequence mutants. The mutated residues are boxed in gray. P6 to P1 and P′1 to P′6 refer to position of the residues relative to the previously characterized or proposed cleavage sites. (C) Arabidopsis protoplasts were transfected with the expression vectors indicated. The cells were harvested 48 h posttransfection, and total protein extracts were subjected to 8% SDS-PAGE and immunoblot analysis with anti-PRR (left) or anti-66K (right). The positions of viral proteins and molecular mass markers are indicated.