FIG. 6.

Detection of the viral helicase in infected cells. Protein extracts from healthy (H) (lane 1) and TYMV-infected (I) (lane 2) Chinese cabbage leaves and from healthy (lane 3) or TYMV-infected (lane 4) Arabidopsis leaves were subjected to SDS-10% PAGE and immunoblot analysis with anti-HEL antiserum. Arabidopsis protoplasts were transfected with water (lane 5), TYMV RNA (lane 6), or the expression vectors indicated. The cells were harvested 48 h posttransfection, and total proteins were subjected to 10% SDS-PAGE and immunoblot analysis with anti-HEL polyclonal antibodies. The positions of viral proteins and molecular mass markers are indicated.