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PRO/HEL and HEL/POL cleavage sequences can be processed in trans in plant cells. Arabidopsis protoplasts were transfected with the expression vectors indicated, either alone or in combination. The cells were harvested 48 h posttransfection, and total protein extracts were subjected to 8% SDS-PAGE and immunoblot analysis with anti-PRR (left) or anti-66K (right) antisera. The positions of viral proteins and molecular mass markers are indicated.
