FIG. 8.

Effects of cleavage site mutations on 206K proteolytic processing. (A) Schematic representation of the 206K protein and mutated derivatives. Residues at the PRO/HEL and HEL/POL cleavage sites are indicated. (B) Arabidopsis protoplasts were transfected with the expression vectors indicated. The cells were harvested 48 h posttransfection, and total protein extracts were subjected to 8% SDS-PAGE and immunoblot analysis with anti-PRR (left) or anti-66K (right) antisera. The positions of viral proteins and molecular mass markers are indicated.