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. 2007 Aug 15;81(20):11372–11380. doi: 10.1128/JVI.01327-07

FIG. 4.

FIG. 4.

Levels of luciferase activity in the photoreceptors after subretinal injections of AAV. (A) Levels of luciferase activity 1 month after subretinal injections of AAV2/5, AAV2/7, AAV2/8, AAV2/9, AAV2/rh.64R1, AAV2/rh.43, and AAV2/rh.29R vectors encoding the bicistronic reporter from the RHO promoter. AAV2/5-CMV-LacZ was coinjected to normalize the variability due to subretinal administrations. The levels of luciferase activity were normalized for β-Gal expression levels. n, number of injected eyes. Differences from AAV2/5 expression levels that are statistically significant are marked with an asterisk; P ≤ 0.05. (B) Fluorescence microscopy evaluation of EGFP expression 4 weeks after subretinal injection of AAV2/5, AAV2/7, AAV2/8, or AAV2/9 vector encoding the bicistronic reporter. (C) Levels of luciferase activity 1 month after subretinal injection of AAV2/5 vectors encoding the bicistronic reporter gene from the CMV, CBA, RHO, or RHOK promoter and AAV2/1-CMV-LacZ. The levels of luciferase activity were normalized for β-Gal expression levels. n, number of injected eyes.