FIG. 5.
Endocytosed replication-competent HIV-1 in DCs does not produce significant amounts of late RT products. (A) HIV-1 fusion is required for generating late RT products in DCs. DCs were infected with HIV-1NLAD8, washed, trypsinized, and cultured for 12 h before the cells were lysed for real-time PCR detection. (B) Enhanced HIV-1 endocytosis and retention in mDC-LPS. DCs were infected with HIV-1NLAD8, washed, trypsinized, and cultured for 12 h before the cells were lysed for Gag p24 detection. T-20 was present during the viral incubation and the 12-h culture. All data show means ± standard deviations for duplicate or triplicate samples. Data for one representative experiment out of three are shown.