TABLE 2.
Frequencies of tags in the L-SAGE librariesa
| No. of occurrences and/or description | No. of tags with unique sequences (total no. of tags) in library generated from:
|
||
|---|---|---|---|
| BCBL-1 cells | Tumor 1811 spiked with BCBL-1 RNA | Combined 1811, 1795 and 448 SCCC data sets | |
| >74 | 2 (198) | 18 (4,124) | 268 (73,198) |
| 25-74 | 31 (1,259) | 59 (2,473) | 648 (25,517) |
| 5-24 | 204 (1,828) | 527 (4,794) | 4,945 (46,089) |
| 2-4 | 757 (1,851) | 1,984 (4,925) | 12,343 (30,926) |
| ≥2 (HiFi) | 994 (5,136) | 2,588 (16,316) | 18,204 (175,730) |
| 1 | 3,688 (3,688) | 7,351 (7,351) | 61,878 (61,878) |
| Unambiguous | 4,682 (8,824) | 9,939 (23,667) | 80,082 (237,608) |
| Total | (9,026) | (23,700) | (241,122) |
a
The total pool of tags was analyzed for tag frequency. Data in italics correspond to the tags identified more than once in the SAGE libraries, which were used as HiFi sequences for DTS analysis.