FIG. 1.

Expression and budding of Rev-dependent and Rev-independent Gag-BiFC constructs in 293T cells. (A) Schematic diagram of plasmids expressing EIAV and HIV-1 Gag-BiFC fusion proteins in either Rev-dependent or Rev-independent manner. An HA epitope indicated by an arrowhead was inserted at the C terminus of p9 or p6 protein. (B) Expression and budding of the constructs depicted in panel A in transfected 293T cells, as described in Materials and Methods. Lanes 1 to 8 in this panel correspond directly to constructs 1 to 8 in panel A. Lanes 9 and 10 were loaded with 1:10 diluted samples loaded in lanes 7 and 8, respectively. At 48 h posttransfection, cell lysates (upper panel) and VLPs (lower panel) were analyzed by immunoblotting using HA antibody.