Figure 5.

Analysis of the neurotrophin receptors of isolated microglia (Microglia) and dorsal root ganglia culture (DRG) by RT-PCR. (A) PCR fragments of the trkA receptor were detected in dorsal root ganglia culture, but not in isolated microglia culture. The p75 neurotrophin receptor was detected in both dorsal root ganglia culture and isolated microglial culture. (B and C) PCR fragments of the trkB and trkC receptors were amplified in isolated microglia culture from the extracellular (extrac.) and intracellular (intrac.) domains. The intracellular tyrosine kinase domains of the receptors were detectable at a very low level. Lanes labeled “Marker” and “Neg.” show the molecular weight marker ΦX174/HaeIII-digested and negative PCR control, respectively.