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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Mol Immunol. 2007 Jul 2;45(1):1–12. doi: 10.1016/j.molimm.2007.05.018

Figure 2.

Figure 2

Figure 2

Figure 2

Figure 2

Mutational analysis of four putative Ets binding sites (EBS) in the Fli1 promoter. A) Sequence alignment showing the homology between the murine (M) and human (H) sequence in exon 1 (-398 to +18). Numbering is relative to the +1 translation start site of the murine sequence. Putative transcription factor binding sites are underlined and indicated above the sequence. The coding region of exon 1 is indicated by a shaded box. The mouse sequence was derived from direct sequencing of gDNA cloned from the BALB/c mouse. The human sequence was obtained from NCBI (Y17293.1). B) Location of putative transcription factor binding sites within the -271/-37 region of the murine sequence. Consensus sequences are underlined and mutations made within each consensus site are indicated by: * EBS1, 2, 3 or 4; ^ EBS2b; # STAT; and $ GATA. C) Transient transfection of pGL3 Fli1 -271/-37 containing mutations within each of the four EBSs into CH1 B cells and S1A T cells. D) Transient transfection of pGL3 Fli1 -271/-37 containing combinations of mutations within three of the four EBSs into CH1 B cells and S1A T cells. Results are representative of at least three independent transfections performed with two independently derived clones.