Figure 1.

TCR induction of protein tyrosine phosphorylation.

Purified naïve, adaptively tolerant, or preactivated T cells were stimulated for 1 min with varying concentrations of anti-TCRβ-biotin mAb (0, 1, or 10μg/ml) and anti-CD4-biotin mAb at either 0 or 10 μg/ml, followed by cross-linking with streptavidin. Samples were lysed with 1% NP40 buffer and run on SDS-PAGE. Western blots were performed and probed with an anti-phosphotyrosine mAb (anti-pTyr) and then reprobed with an anti-actin mAb.