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. Author manuscript; available in PMC: 2007 Oct 30.
Published in final edited form as: Semin Immunol. 2007 Apr 2;19(3):140–152. doi: 10.1016/j.smim.2007.02.005

Figure 4.

Figure 4

cCbl phosphorylation.

Purified naïve, adaptively tolerant, or preactivated 5C.C7 T cells in (A) or control and anergized A.E7 T cells in (B) were stimulated for 1 min with varying concentrations of anti-TCRβ-biotin mAb (0, 1, or 10μg/ml) and anti-CD4-biotin mAb at 10 μg/ml, followed by cross-linking with streptavidin. Samples were lysed with 1% NP40 buffer, immunoprecipitated with anti-cCbl antiserum, and run on SDS-PAGE. Western blots were performed and probed with anti-phosphotyrosine mAb and then reprobed with anti-cCbl mAb.