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. Author manuscript; available in PMC: 2007 Oct 30.
Published in final edited form as: J Biol Chem. 2002 Sep 18;277(48):45984–45991. doi: 10.1074/jbc.M200250200

Fig. 7. ΔhPPAR-α does not block gemfibrozil-mediated inhibition of iNOS promoter activation in cytokine-stimulated human U373MG astroglial cells .

Fig. 7

Cells were cotransfected with 0.5 μg of either ΔhPPAR-α or an empty vector, 0.5 μg of phiNOS(7.2)Luc, and 50 ng of pRL-TK. Twenty-four hours after transfection, cells were incubated with gemfibrozil for 2 h followed by stimulation with the combination of IL-1β and IFN-γ. After 12 h of incubation, firefly (ff-Luc) and Renilla (r-Luc) luciferase activities were assayed. Data are mean ± S.D. of three different experiments.