Fig. 3.
VIP mRNA in CE cells from donor human corneas stored in Optisol. RT-PCR product (A) and real-time RT-qPCR product (B) of VIP mRNA were electrophoresed (2% agarose). For A- Rgnt: reagent negative control, RT-: RNA not subjected to RT, STD: DNA standard, bp: base pair, NBFL: human neuroblastoma cell line as positive control. CE 86 bp product was sequenced. B: In the presence of SYBR green, the RT product of CE RNA was subjected to PCR.