Fig. 4.
CNTF- induced VIP mRNA in CE cells in stored donor human corneas demonstrated by real-time RT-qPCR. VIP mRNA level over that of the endogenous mRNA standard (cyclophilin B or β- actin) found in each of the two corneal halves (control vs CNTF-treated) of the same cornea was normalized against that of the control half and values derived from both the control and the CNTF-treated halves were given on Y-axis. CNTF treatment (0.1 nM, 24 h) increased VIP mRNA levels by (mean±SEM) 6.54±2.25 (N=7 corneas, p=0.024; cyclophilin B) and 2.32±0.59 (N=10 corneas, p=0.019; β-actin) folds.