Table 2.
Primers for subcloning β-crystallin genes to GFP and DsRED vectorsa
| Forward primers | Reverse primers | |
|---|---|---|
| βA2 | TTTTCTCGAGTGAGCAGCGCCCCCGC | CGAATTCCTAGTGCTGGACTCTCC |
| βA4 | AACAAGCTTTGACCCTGCAATGC ACA | GGAATTCTCACTGCTGGATCCTGCG |
| βB2 | GAACCTCGAGCTGCCTCAGATCACCAG | GGTCTAGAATTCCTAGTTGGAGGGGTG |
The underlined sequences are restriction enzyme sites for 5′ and 3′ primers, XhoI and EcoRI, respectively, for βA2 and ββ2. The corresponding restriction enzyme sites for βA4 are HindIII/EcoRI.