. Author manuscript; available in PMC: 2007 Oct 31.

Published in final edited form as: FEBS Lett. 2007 Jul 23;581(21):3936–3942. doi: 10.1016/j.febslet.2007.07.022

Table 3.

Primers for cloning of β-crystallin genes to expression pQE30 vector^a

	Forward primers	Reverse primers
βA1	TTTGGATCCGCTCAGACCAACCCTACC	TTTGGTACCCTACTGTTGGATTCGGCG
βA2	TTTGGATCCTTGAGCAGCGCCCCCG	TTTGGTACCCTAGTGCTG GACTCT
βA3	TTTGGATCCGAGACCCAGGCTGAGCAG	TTTGGTACCCTACTGTTGGATTCGGCG
βA4	AAAGGTACCACCCTGCAATGCACA	CCCAAGCTTTCACTGCTGGATCCT
βB1	TTTGGATCCTCTCAG GCTGCAAAG GCC	TTTAAGCTTTCACTTGGGGGGCTC
βB2	CGGGGTACCCCGGCCTCAGATCACCAG	CCCAAGCTTGGGGTTGGAGGGGTGGAA
βB3	AAAGGTACCGCGGAACAGCACGGA	AAGAAGCTTTCAGCTGCTGGGGAA

The underlined sequences are restriction enzyme sites for 5′ and 3′ primers, respectively: BamHI/KpnI for BA1-,BA2-, and βA3-crystallin; KpnI/HindIII for βA4-, βB2-, and βB3-crystallins; and BamHI/HindIII for βB1-crystallin.