Figure 4.

Aβ42 activates NFκB at p65/RelA TAD 2. NFκB(1)-Gal4 and NFκB(2)-Gal4 expression constructs containing the first and second TADs of p65/RelA, respectively, were individually cotransfected with the yeast Gal4-UAS luciferase reporter plasmid to determine from which domain NFκB activation by Aβ42 occurred. Ten micromolar Aβ42-stimulated astrocytes showed approximately 2- to 3-fold more NFκB(2)-Gal4 luciferase activity compared with PBS-treated or 10 μM Aβ42scr-treated cells. There was no significant stimulation of NFκB(1)-Gal4 in Aβ42-treated cells compared with PBS-treated or 10 μM Aβ42scr-treated cells. ∗, Significantly different from PBS control (P < 0.005).