Table 1.
Days at 21°C post-cold treatment | Methylation level | |
---|---|---|
Unvernalized | Vernalized | |
0 | 83.3 ± 1.6 | 74.0 ± 2.5 |
4 | 80.3 ± 2.4 | 77.7 ± 3.0 |
7 | 86.7 ± 3.3 | 83.7 ± 3.2 |
14 | 102.1 ± 3.6 | 95.2 ± 3.5 |
21 | 95.8 ± 1.8 | 98.0 ± 3.8 |
Methylation level is expressed as % ± SE, relative to a standard, isolated from mature leaves. Methylation estimates were done at least three times for each sample. The difference in methylation between cold-treated and untreated seedlings was replicated in six independent experiments; the recovery of methylation was observed in two independent experiments.