Figure 2.

Blot panel of 29 maize-specific cosmid clones isolated from a cosmid library of an oat–maize chromosome 9 addition line. (a) Cosmids (1–29) are cut by EcoRI restriction enzyme and size-fractionated in an 0.85% agarose gel stained with EtdBr (M = molecular weight marker, 1-kb ladder). (b) Labeled, total genomic maize DNA as a probe shows strong, medium, or weak hybridization to almost 90% of all EcoRI subfragments present in the cosmid clones. (c) Labeled multiprobe of highly repeated maize DNA sequences shows hybridization to from one to five EcoRI fragments in each lane.