Figure 3.

Sequence comparison of six modified clones with that of genomic DNA. WT, sequence of the wild-type polyoma genome. #1–#6, sequences of the modified clones. Only the modified bases are shown in the six clones; other sequences were identical, except as noted. Lowercase letters indicate primer binding sites. The EcoR1 site is underlined, and the percentage of the modified adenosines is indicated for each sequence. A synthetic DNA oligodeoxynucleotide complementary to the sequence within the boxed region was used for the experiments described in Fig. 4.