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Evaluation of CGP64222 in the FIGS assay. Tat activity was assessed 24 hr after coculture of chronically HIV-1-infected HUT4–3 cells with SX22–1 indicator cells. (×1000.) (A) Untreated control cells showing syncytium formation and the subsequent activation of the lacZ gene by Tat. (B) Cells treated with 10 μM Arg5 peptide. Note that fusion is inhibited. (C) Cells treated with 10 μM CGP64222. Note that generation of syncytia is normal, but Tat activity is completely suppressed.
