Table 4.

Results of Replicate Automated Purification of His-TEV237Δ.

Purification cycle	Final Volume mL	Protein Concentration mg/mL	Total Protein mg	Total Activity μmol/hr	Specific Activity μmol/h/mg
Cycle 1	20	4.91 ± 0.16	98 ± 3	33 ± 6	0.34 ± 0.06
Cycle 2	20	5.03 ± 0.26	101 ± 5	34 ± 8	0.34 ± 0.08
Cycle 3	20	4.97 ± 0.22	99 ± 4	34 ± 2	0.34 ± 0.02
Cycle 4a	20	4.85 ± 0.05	97 ± 1	32 ± 6	0.33 ± 0.06
Cycle 5	20	5.02 ± 0.21	100 ± 4	33 ± 3	0.33 ± 0.03
Cycle 6b	10	1.05 ± 0.01	10 ± 0.1	2.4 ± 0.4	0.23 ± 0.04

^aIn cycle 4, the imidazole concentration in the wash buffer was 100 mM.

^bIn cycle 6, the remaining lysate was used. In addition, the imidazole concentration in the wash buffer was increased to 125 mM.