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. Author manuscript; available in PMC: 2008 Sep 1.
Published in final edited form as: Protein Expr Purif. 2007 Apr 25;55(1):53–68. doi: 10.1016/j.pep.2007.04.013

Table 5.

Purification Of His7-TEV Protease After Auto-Induction Of Expression From Vector pMHT237Δ In A 10-L Fermenter.

Sample Volume Total Protein Total Activity Recovery Specific Activity Fold-purification
mL mg μmol/h % μmol/h/mg
Cell-free lysatea 168 3120 156 100 0.05 1
IMAC wasteb 740 2860 1.3 1 0.0005 0.01
Cation exchange wastec 1830 15 2.4 2 0.16 3.2
Purified His7-TEVd 80 395 133 85 0.34 6.7
a

The lysate obtained from 25 g of MHT237Δ cells.

b

Collected as flow-through from sample injection and column wash prior to elution.

c

Collected as flow-through from sample injection and column wash prior to elution.

d

Pooled sample obtained after four cycles of the 2-step automated purification.