Figure 4.

Presence of secretin gene E-box binding factors in different cell types. (A) Gel shift analysis of nuclear extracts from the indicated cell lines using the wild-type probe. Position of complex 1 is indicated by an arrowhead. Complex 1 was present in the two endocrine cell lines, HIT and STC1, but not in HeLa or 3T3 cells. (B) Autoradiogram from a separate gel similar to A. The first lanes show the position of complex 1 generated by HIT nuclear extracts without antibody. Complex 1 from each of the endocrine cell lines is entirely immunoneutralized with antibodies against BETA2. Cell lines include STC1, 3T3 (murine), HIT, (hamster), and HeLa (human).