Figure 5.

BETA2 activates the secretin enhancer in both endocrine and nonendocrine cells. Cells were transfected with the secretin-luciferase reporter gene. Expression plasmids for shPan1, shPan2, BETA2, and antisense BETA2 were cotransfected as indicated. (A) Secretin-producing HIT cells (solid bars) and STC-1 (striped bars). (B) HeLa cells. Results are expressed as relative luciferase activity generated compared with the reporter alone and are the mean ± SEM of six independent transfections for each construct. Values significantly different from cells transfected with the reporter gene alone are denoted by ∗, P < 0.05; ∗∗, P < 0.01; and ∗∗∗, P < 0.001.