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. Author manuscript; available in PMC: 2008 Oct 1.
Published in final edited form as: Pharmacol Ther. 2007 Apr 20;116(1):35–57. doi: 10.1016/j.pharmthera.2007.03.004

Figure 4. Effect of cyclodextrin on the washout of currents and fluorescence of a tagged steroid analogue.

Figure 4

A1. NBD-3α5αP (300 nM) was applied to neurons in the absence of GABA, and whole-cell currents were monitored. Drug exposure is indicated by the horizontal bar. Despite rapid removal of free drug, the current decayed slowly. A2. Including 500 μM γ-cyclodextrin (CDX) in the wash following drug removal (solid trace) increased the rate of current offset. The dotted trace is a re-plot of A1. B1 and B2. Imaging of a neuron following exposure to fluorescent steroid analogue at the time points denoted by the small letters in A2. The fluorescence intensity is pseudocolored, with warm colors representing high fluorescence intensity. B1 shows loss of fluorescence with normal saline wash. B2 shows intracellular fluorescence loss is speeded by cyclodextrin wash. The figure is taken from (Akk et al., 2005). Copyright Society for Neuroscience.