Table 1.

Batchwise renaturation of cyclophilin A on mini-chaperone-agarose gels

Gel type	Protein yield, %	Activity yield, %	Specific activity relative to “pure” native, %
GroEL(191–345)-Ni-NTA-agarose	84	98	103
GroEL(191–376)-Ni-NTA-agarose	81	105	115
GroEL(191–345)-agarose	87	125	126
HSA-agarose (control)*	84	33	35
Ethanolamine-agarose (control)†	80	25	28
Agarose (control)‡	72	17	20
Native cyclophilin (control)			88§

Cyclophilin activity was measured in the supernatant as described (12). 

^*Human serum albumin (HSA) was covalently linked to CNBr-activated agarose. 

^†CNBr-activated agarose was coupled to ethanolamine. 

^‡NTA-agarose after removal of the Ni from Ni-NTA agarose. 

^§The sample prior to denaturation had 88% of the specific activity of the highest activity of native cyclophilin previously obtained in this laboratory.