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. Author manuscript; available in PMC: 2008 Oct 12.
Published in final edited form as: J Mol Biol. 2007 Aug 10;373(1):178–189. doi: 10.1016/j.jmb.2007.07.062

FIGURE 5.

FIGURE 5

Cleavage of a short oligonucleotide in trans. (a) Reaction time course for the wild-type sequences. Cleavage products of the 5' end-labeled substrate 7-mer RNA were fractionated on PEI plates in 1M LiCl. The product comigrated with a pAp marker generated by nuclease U2 digestion (not shown). (b) The released 5' nucleotide contains a 2',3'-cyclic phosphate group. Treatment with HCl caused the product of the cleavage reaction to migrate slower at pH 5 (1M LiCl with 0.1M Boric acid pH 5). This result is consistent with a 5' product that contains a cyclic phosphate that is hydrolyzed by acid treatment.