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Long-lasting anti-Aβ antibody responses in mice immunized with 5 μg of Aβ28 conjugated with mannan (the data with similar profile were obtained with 2.5 and 10 μg mannan-Aβ28). After vaccination and three booster injections mice were rested for 2 months, and the immune response was recalled with the appropriate antigen. Individual sera from animals were diluted 1:250 and tested for detection of anti-Aβ42 antibodies in ELISA. The experiment was repeated with similar results.
