FIG. 5.

Mannan–Aβ₂₈ and fibrillar Aβ₂₈-induced Th2-type cellular immune responses in BALB/c mice. (A) Immune splenocytes isolated from individual mice immunized with mannan–Aβ₂₈ or fibrillar Aβ₂₈ and in vitro restimulated by Aβ₄₀ peptide-induced robust T-cell proliferation. Data are presented as Stimulation Index (SI). (B) Production of Th1 (IFNγ) and Th2 (IL-4)-type cytokines, as well as pro-inflammatory TNFα by immune splenocytes isolated from mice immunized with 5 μg of mannan–Aβ₂₈ or fibrillar Aβ₂₈. The ELISPOT technique was used as described in Materials and Methods, and data are presented as a delta between number of spots in activated with Aβ₄₀ and nonactivated splenocyte cultures.