FIG. 6.

Mannan–Aβ₂₈, as well as fibrillar Aβ₂₈ induced predominantly antibodies specific to the N-terminal region of Aβ₄₂. Mapping of B-cell epitopes was conducted by competition ELISA as we previously described (Cribbs et al., 2003b). Individual sera from immune mice (final dilution 1:250) were collected and preincubated with Aβ_1–15 Aβ_6–20, Aβ_11–25, Aβ_16–30, or Aβ₄₂ peptides with the final concentration of each peptide being 2.5 μM before binding to Aβ₄₂-coated wells. Representative ELISA data from two experiments.