Figure 1.

Construction and expression of SF2/ASF-SC35 chimeric proteins. (A) Wild-type SF2/ASF and SC35. Domains of SF2/ASF and SC35 are shown by open and shaded boxes, respectively (not to scale). Three restriction sites shown in the wild-type constructs were used to swap domains between the two proteins. The nomenclature is based on the origin of each domain: F, a domain from SF2/ASF; C, a domain from SC35. RRM, RNA-recognition motif. RS, arginine/serine-rich domain. (B) Domain-swap proteins. (C) Domain-deletion proteins. (D) Purified GST-fusion proteins resolved by 12.5% SDS/PAGE. Molecular weight markers are indicated on the right.