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Activities of domain-deletion mutants in the splicing commitment assay. (A) Human β-globin pre-mRNA. (B) HIV tat pre-mRNA. Each pre-mRNA was spliced in the absence (lane 1) or presence (lanes 2–7) of unlabeled competitor RNA after preincubation under splicing conditions with buffer control (lanes 1 and 2) or with wild-type and domain-deletion mutants (lanes 3–7), as indicated above the autoradiogram.
