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. 2007 Nov 2;3(11):e198. doi: 10.1371/journal.pgen.0030198

Figure 3. Immunolocalization of SYCP3 (Red) and RAD51 (Green) (A–C) and SYCP3 (Red) and MLH1 (Blue) (D) Proteins on Spread Spermatocytes.

Figure 3

(A–A′) At early pachytene RAD51 foci are visible over the autosomes. This protein also appears associated to the AEs of sex chromosomes (enlarged in A′).

(B–B′) At mid pachytene the number of RAD51 foci over the autosomes and sex chromosomes decreases, but RAD51 begins to accumulate over the sex chromatin as a diffuse signal.

(C–C′) At late pachytene RAD51 is absent from autosomes but intensely extended on the sex chromatin.

(D) MLH1 protein is detectable at late pachytene as one single dot over most of the autosomal axes, although some bivalents present two (arrowheads). No signal of this protein is detected over the axes of the sex chromosomes (D′).