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. Author manuscript; available in PMC: 2008 Jun 15.
Published in final edited form as: Neuroscience. 2007 May 23;147(1):164–173. doi: 10.1016/j.neuroscience.2007.02.063

Figure 4. Expression of NR1 splice variants in the rat colon.

Figure 4

(A) Expression of N1 cassette (exon 5) in the rat colon. RT-PCR analyses of N1 cassette (exon 5) expression in rat colon and brain tissues using four different sets of primers; “NR1a(b)”, “exon 5+”, “N1+” and “N1−”. (B) Western Blot showing expression of N1 cassette containing NR1 protein in GI and brain tissues using antibody against N1. (C) Expression of C1 cassette (exon 21) in the rat colon. RT-PCR results of C1 cassette (exon 21) expression in rat colon and brain tissues using two different set of primers; C1 and C1+. (D) Western blot showing expression of C1 cassette containing NR1 protein using antibody targeted towards the C-terminal of the C1 cassette (exon 21). (E) Expression of C2 cassette (exon 22) in the rat colon. RT-PCR results of C2 cassette (exon 22) expression in rat colon and brain tissues using primers targeted to the C2 cassette. (F) Western blot showing probing of C2+ antibody which is targeted to the C2 cassette and C2− antibody directed against the C2′ cassette.