Table 1. RT-PCR Primers Sequences.

All primers were purchased from GenoMechanix (Gainesville, Fl). RT-PCR reactions were carried out using Access RT-PCR System from Promega (Madison, WI) with the following cycle conditions: 1 cycle at 48°C for 45 min, 94°C 27 for 2 min and 72°C for 1 min, 35 cycles of PCR (94°C for 30 sec, 60°C for 1 min, 72°C for 2 min) and a final elongation period of 7 min at 72°C.

Primer name	Forward sequence	Fwd Tm	Reverse sequence	Rev Tm	Product length
General
NR1 general	cagaaacccctcagacaagttc	59°C	cttctgtgaagcctcaaactcc	59°C	213bp
NR1 generic	ccctcagacaagttcatctacgc	61°C	aggttcttcctccacacgttcac	61°C	563bp
GAPDH	ccttcattgacctcaactacatggtcta	63°C	tagcccaggatgcccttt	55°C	720bp
Exon 5 or N1
NR1a Exon 5 (−)	gcgagtctacaactggaaccac	61°C	ctcgcttgcagaaaggatgatg	59°C	170bp
NR1b Exon 5 (+)					235bp
Exon 5+ inside	ggaactatgaaaacctcgacc	58°C	NR1b Exon 5 Reverse	59°C	158bp
N1+ (ζ1 N1+)	accacttcactcccacccctgtctcctac	72°C	gtccgcgcttgttgtcata	61°C	330bp
N1− (ζ1 N1−)	agctcaacgccacttctgtc	61°C	caccttctctgccttggactcac	65°C	407bp
Exon 21 or C1
C1	UseNR1 general Fwd or	59°C	gttttgcaaagcgccgcgtcca	63°C	717bp
	NR1 generic Fwd	61°C			710bp
C1+ (ζ1 C1+)	tgtgtccctgtccatactcaag	60°C	gtcgggctctgctctaccac	62°C	307bp
Exon 22 or C2
NR1 Exon-22	catggcaggggtcttcatgctg	63°C	gaacacagctgcagctggccct	65°C	318bp
NR2
NR2A	tatagagggtaaatgttgga	50°C	agaaactgtgaggcatttct	52°C	322bp
NR2B	actgtgacaacccacccttc	58°C	cggaactggtccaggtagaa	58°C	400bp
NR2C	tgtgtcaggccttagtgaca	56°C	ccacactgtctccagcttct	58°C	402bp
NR2D	aagaagatcgatggcgtctg	56°C	ggatttcccaatggtgaagg	56°C	353bp

All primers from GenoMechanix, Gainsville, Fl, USA.