Table 2. Antibodies used for Western Blots and Immunohistochemical Assays.
Antibodies were purchased from different vendors and dilutions were prepared according to vendors’ specifications. Incubation time for primary antibodies was 24 hours and 1 hour for secondary antibodies.
| Antibody | Host | Company | Cat. No. |
|---|---|---|---|
| Primary Antibodies | |||
| Actin | Mouse | Chemicon, Temecula, CA. | MAB1501 |
| Neurofilament | Mouse | Sigma, Saint Louis, Missouri. | N-5389 |
| NR1 | Mouse | BD Biosciences Pharmigen, San Diego, CA. | 556308 |
| NR1 | Rabbit | Santa Cruz Biotechnology, Santa Cruz, CA. | SC-9058 |
| NR1 splice variants | Rabbit | Provided by Dr. Michael Iadarola, The National Institute of Dental and Craniofacial Research NIDCR, Bethesda, MD. | N/A |
| NR2B | Rabbit | Santa Cruz Biotechnology, Santa Cniz, CA. | SC-9057 |
| NR2B | Mouse | BD Biosciences Pharmigen, San Diego, CA. | 610417 |
| NR2D | Rabbit | Santa Cruz Biotechnology, Santa Cruz, CA. | SC-10727 |
| Protein Gene Product 9.5 | Rabbit | Chemicon, Temecula, CA. | AB1761 |
| Fluorescent Secondary Antibodies | |||
| Alexa Fluor 488 | Mouse | Molecular Probes, Eugene, OR. | A21202 |
| Alexa Fluor 488 | Rabbit | Molecular Probes, Eugene, OR. | A21206 |
| Alexa Fluor 594 | Mouse | Molecular Probes, Eugene, OR. | A21201 |
| Alexa Fluor 594 | Rabbit | Molecular Probes, Eugene, OR. | A21442 |