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. 1997 Apr 15;94(8):3602–3604. doi: 10.1073/pnas.94.8.3602

Figure 1.

Figure 1

Figure 1

The distant BXD enhancer and a proximal promoter element of Ubx interact synergistically to drive high levels of expression in the Drosophila embryo. (A) Expression of the Uβ promoter construct that contains the BXD and ABX enhancer elements and the intact proximal promoter of Ubx (D). (B) Expression of promoter construct Uβ Δ−200/−31, which is identical to Uβ except that promoter elements between nucleotides −200 and −31 have been deleted (D). (C) Expression of ssDpry promoter construct, which is identical to Uβ, except that it lacks the BXD enhancer (D). (D) Schematic representations of the Ubx promoter constructs are diagrammed. Ubx promoter DNA between nucleotides −3.1 kb and +1 kb is drawn as a horizontal line, and the ABX and BXD distant regulatory elements and the Escherichia coli lacZ gene are boxed. The start site of transcription is denoted by an arrow. The extent of the deletion at the proximal promoter in Uβ Δ−200/−31 is also indicated. This deletion removes the five zeste DNA binding sites in this region.