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Treatment with PACAP or forskolin has no effect on VIP expression in cultured cardiac neurons. Cardiac ganglia explants were cultured for 24 hours (A) or 72 hours (B) in the presence of PACAP (20 nM) or forskolin (10 μM) or without either drug. Neither treatment affected the percentage of VIP-IR neurons. At least 3 preparations were analyzed under each condition.
