Fig. 1.

Cyanide-induced cell death and ROS generation. (A) Cells were treated with varying concentrations of KCN (200–600 μM) for 24 h, followed by analysis of cell viability by trypan blue exclusion test. (B) Cells were treated with KCN (200–600 μM) for varying times (0–24 h), followed by analysis of apoptosis by Hoechst 33258 staining. (C) Cells were pretreated with NAC (0.5 mM), CAT (0.2 mM), SB203580 (20 μM) or SB202474 (20 μM) for 30 min prior to incubation with KCN (400 μM) for 30 min, then ROS generation determined. (D) KCN-induced ROS generation was determined in the presence or absence of FCCP (1 μM). (E) Apoptotic death was determined in the presence or absence of different concentrations of NAC or CAT for 30 min and then KCN (400 μM) treatment for 24 h. Data represent mean ± SEM of 3–8 separate determinations. *Significantly different from control (no treatment) group; #significantly different from KCN (control) group. P<0.05