Fig. 2.

Cyanide-induced activation of p38 MAP kinase. (A) Cells were treated with 400 μM cyanide (0.5–4 h), and whole cell lysates subjected to Western blot analysis with anti-phospho-p38 MAP kinase and anti-p38 MAP kinase antibodies. (B) Blockade of cyanide-induced activation of p38 MAP kinase. Cells were pretreated for 30 min with NAC (0.5 mM), CAT (0.2 mM), SB203580 (20 μM) or SB202474 (20 μM), then with 400 μM KCN for 30 min. p38 and phospho-p38 MAP kinase proteins were determined by Western blotting. Densitometric analysis is the mean ± SEM of three separate determinations. *Significantly different from control group; #significantly different from KCN group. P<0.05.